4株H5N6亚型禽流感病毒毒株的分离鉴定与致病性研究

doi:10.11843/j.issn.0366-6964.2018.01.017

Abstract

Abstract:

The purpose of this study was to investigate the biological characteristics of 4 H5N6 subtype avian influenza virus isolates, and their pathogenicity to chickens. In this study, the clinical samples between 2015-2016 year in Guangdong were used to isolate virus, and the isolates were identified, the genes of isolates were cloned, sequenced and analyzed, then 4 weeks old SPF chickens were infected by the way of intranasal and eyedrop inoculation. Our results showed that 4 strains of H5N6 avian influenza virus were isolated, their HA gene belonged to Clade 2.3.4.4, and the isolates contained multiple basic amino acids at the cleavage site, which were characteristic of highly pathogenic AIV. The inoculation results showed that all chickens of the attack group died in 5 d, and the chickens of cohabitation group died in 9 d; the infected chickens could shed virus continuously. And the avian influenza virus could be detected in the heart, liver, spleen, lung, kidney, brain in high titer and caused extensive pathological damage. The 4 strains of H5N6 subtype avian influenza virus isolates showed high pathogenicity and horizontal transmission ability to chickens, suggesting the need to strengthen the prevention and control of H5N6 subtype avian influenza.

CLC Number:

S852.659.5

ZHOU Pei-jiao, XING Li, JIA Wei-xin, LIAO Ming. Isolation,Identification and Pathogenicity Analysis of Four H5N6 Avian Influenza Virus Isolates[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 147-156.

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Isolation,Identification and Pathogenicity Analysis of Four H5N6 Avian Influenza Virus Isolates

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